Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

Group-Specific Identification of Polioviruses by PCR Using Primers Containing Mixed-Base or Deoxyinosine Residues at Positions of Codon Degeneracy

Codon Degeneracy or Deoxyinosine Residues at Positions of by PCR Using Primers Containing Mixed-Base Serotype-Specific Identification of Polioviruses

Type-specific detection of echovirus 30 isolates using degenerate reverse transcriptase PCR primers.

Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These primers permit specific RT-PCR amplification of echovirus 30 (E30) sequences by targeting sites that encode conserved amino acid motifs within the major capsid protein,...

Association of Necrotizing Wounds Colonized by Maggots with Ignatzschineria–Associated Septicemia

References 1. Kew OM, Sutter RW, de Gourville EM, Dowdle WR, Pallansch MA. Vaccine-derived polioviruses and the endgame strategy for global polio eradication. Annu Rev Microbiol. 2005; 59:587–635. http://dx.doi.org/10.1146/ annurev.micro.58.030603.123625 2. World Health Organization. Supplement to the WHO polio laboratory manual. An alternative test algorithm for poliovirus isolation and charac...

Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Multiplex bisulfite-PCR sequencing is a convenient and scalable method for the quantitative determination of the methylation state of target DNA regions. A challenge of this application is the presence of CpGs in the same region where primers are being placed. A common solution to the presence of CpGs within a primer-binding region is to substitute a base degeneracy at the cytosine position. Ho...